Journal: OncoTargets and therapy

Article Title: Long Noncoding RNA LINC01485 Promotes Tumor Growth and Migration via Inhibiting EGFR Ubiquitination and Activating EGFR/Akt Signaling in Gastric Cancer

doi: 10.2147/OTT.S257151

Figure Lengend Snippet: Microarray analysis of differentially expressed lncRNAs and mRNAs in gastric cancer. ( A ) Heat map and hierarchical clustering analyzed the distinguishable lncRNA ( A ) and mRNA ( B ) profiling in gastric cancer tumor tissues and paratumor counterparts. The relative expression from high to low level was indicated with red and blue color. ( C and D ) The expression levels of 6 upregulated candidate mRNAs and 8 upregulated novel candidate lncRNAs were validated in 20 paired gastric cancer tumor tissues and paratumor counterparts with RT-PCR. The data were presented as the mean ± SEM, * P < 0.05, Student’s t -test. ( E ) The specific gene-expression patterns were determined by weighted gene co-expression network analysis (WGCNA). ( F ) The correlation analysis of EGFR and 4 lncRNAs were examined in 20 gastric cancer tumor tissues with RT-PCR. Abbreviations: lncRNA, long non-coding RNA; RT-PCR, real-time polymerase chain reaction; SEM, standard error of the mean; EGFR, epithelial growth factor receptor.

Article Snippet: The lncRNA/mRNA crosslinked microarray was employed (Capitalbio, Beijing, China).

Techniques: Microarray, Expressing, Reverse Transcription Polymerase Chain Reaction, Gene Expression, Real-time Polymerase Chain Reaction

Journal: BioMed Research International

Article Title: Diagnostic and Prognostic Value of Long Noncoding RNAs as Potential Novel Biomarkers in Intrahepatic Cholestasis of Pregnancy

doi: 10.1155/2021/8858326

Figure Lengend Snippet: Primer sequences used in validation of lncRNAs.

Article Snippet: We got the mRNA and lncRNA microarray data from CapitalBio Corp. (Beijing, China).

Techniques: Biomarker Discovery

Journal: BioMed Research International

Article Title: Diagnostic and Prognostic Value of Long Noncoding RNAs as Potential Novel Biomarkers in Intrahepatic Cholestasis of Pregnancy

doi: 10.1155/2021/8858326

Figure Lengend Snippet: The lncRNA expression profile in ICP compared with controls. (a) Differently expressed lncRNAs by hierarchical clustering analysis. Red represents upregulated genes, green represents downregulated genes, and the difference is more than 2 times. (b) Different expression of lncRNAs by scatter plot. Red represents upregulated genes, green represents downregulated genes, and the difference is more than 2 times. (c) Different expression of lncRNAs by volcano plot. Red represents upregulated genes, green represents downregulated genes, and the difference is more than 2 times.

Article Snippet: We got the mRNA and lncRNA microarray data from CapitalBio Corp. (Beijing, China).

Techniques: Expressing

Journal: BioMed Research International

Article Title: Diagnostic and Prognostic Value of Long Noncoding RNAs as Potential Novel Biomarkers in Intrahepatic Cholestasis of Pregnancy

doi: 10.1155/2021/8858326

Figure Lengend Snippet: Part of differentially expressed lncRNAs in the serum from pregnant women with ICP (P) compared to healthy pregnant women (C).

Article Snippet: We got the mRNA and lncRNA microarray data from CapitalBio Corp. (Beijing, China).

Techniques:

Journal: BioMed Research International

Article Title: Diagnostic and Prognostic Value of Long Noncoding RNAs as Potential Novel Biomarkers in Intrahepatic Cholestasis of Pregnancy

doi: 10.1155/2021/8858326

Figure Lengend Snippet: The correlation of serum lncRNA level and TBA in ICP patients. (a) ENST00000505175.1 ( R = 0.401, P < 0.0001), (b) ASO3480 ( R = 0.319, P = 0.0009), and (c) ENST00000449605.1 ( R = 0.300, P = 0.0018).

Article Snippet: We got the mRNA and lncRNA microarray data from CapitalBio Corp. (Beijing, China).

Techniques:

Journal: Thoracic Cancer

Article Title: LncRNA and mRNA expression associated with myasthenia gravis in patients with thymoma

doi: 10.1111/1759-7714.14201

Figure Lengend Snippet: Plots of microarray analysis data. Hierarchical cluster analysis plots of the expression of (a) long non‐coding RNAs (lncRNAs) and (b) messenger RNAs (mRNAs) in thymomas of patients with and without myasthenia gravis. Red denotes high relative expression, and green denotes low relative expression. Each RNA is represented by a single row of colored boxes, and each sample is represented by a single column. The three‐dimensional principal component analysis plots for normalized data of (c) lncRNAs and (d) mRNAs, respectively, show good similarity among groups

Article Snippet: LncRNA/mRNA microarray experiments were performed by CapitalBio Technology Corporation, Beijing, People's Republic of China.

Techniques: Microarray, Expressing

Journal: Thoracic Cancer

Article Title: LncRNA and mRNA expression associated with myasthenia gravis in patients with thymoma

doi: 10.1111/1759-7714.14201

Figure Lengend Snippet: Comparison of long non‐coding RNA (lncRNA) and messenger RNA (mRNA) expression in patients with thymoma with myasthenia gravis [MG(+)] and patients with thymoma without MG [MG(−)] by real‐time polymerase chain reaction (PCR). The selected lncRNAs and mRNAs were validated by real‐time PCR of RNAs extracted from five MG(+) thymomas and 3 MG(−) thymomas. Each sample was analyzed in triplicate. Column heights represent mean fold changes in expression in the MG(−) group. The real‐time PCR results are consistent with microarray data

Article Snippet: LncRNA/mRNA microarray experiments were performed by CapitalBio Technology Corporation, Beijing, People's Republic of China.

Techniques: Comparison, Expressing, Real-time Polymerase Chain Reaction, Microarray

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway

doi:

Figure Lengend Snippet: Analysis of long non-coding RNAs (lncRNAs) in oral squamous cell carcinoma (OSCC). A: Heat map, volcano plot, and log-log scatter plot showing differentially expressed lncRNAs between four OSCC samples and paired adjacent normal tissues (fold change > 2.0, P < 0.05). B: The expression of lncRNA P4713 was detected by quantitative real-time PCR (qRT-PCR) in 22 OSCC tissues and adjacent non-cancerous tissues. The results are expressed as log10 (2-ΔΔCt). A log2 fold change ≥ +2 or ≤ -2 was considered significant upregulation or downregulation (dotted lines). C: Relative P4713 expression in OSCC cell lines was measured by qRT-PCR. Columns represent the mean of three independent experiments; bars, the s.d; *P < 0.05; **P < 0.01. D: Confocal microscopic fluorescent in situ hybridization images and qRT-PCR results. Scale bar = 10 µm. E: Genome location analysis of human P4713 by the UCSC Genome Browser. F: Representative fluorescent images of at least three independent experiments. Scale bar = 10 µm. G: Relative levels of GFP expression by Western blot.

Article Snippet: Microarray analysis The OSCC lncRNA/mRNA microarray analysis was performed by CapitalBio Corporation (Beijing, China).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, In Situ Hybridization, Western Blot

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway

doi:

Figure Lengend Snippet: The effects of P4713 on oral squamous cell carcinoma cell proliferation in vitro. A: The relative expression of P4713 was examined by qRT-PCR in HSC-3 and UM1 cells. B: Cell proliferation was measured by CCK-8 assay. C: Detection for colony-formation assays after knockdown of P4713. D: Cell cycle analysis using propidium iodide staining. E: Western blot analysis of cyclin D1, CDK4, and CDK6 after P4713 knockdown.

Article Snippet: Microarray analysis The OSCC lncRNA/mRNA microarray analysis was performed by CapitalBio Corporation (Beijing, China).

Techniques: In Vitro, Expressing, Quantitative RT-PCR, CCK-8 Assay, Knockdown, Cell Cycle Assay, Staining, Western Blot

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway

doi:

Figure Lengend Snippet: Silencing of P4713 suppressed the migration and invasion of oral squamous cell carcinoma (OSCC) cells. A: Inhibition of migration in HSC-3 and UM1 cells after P4713 knockdown. B: A Matrigel invasion assay was performed using an invasion chamber after treatment with si-P4713. C: In vitro migration was assessed by wound healing experiments. D: E-cadherin, N-cadherin, and vimentin were analyzed by western blotting.

Article Snippet: Microarray analysis The OSCC lncRNA/mRNA microarray analysis was performed by CapitalBio Corporation (Beijing, China).

Techniques: Migration, Inhibition, Knockdown, Invasion Assay, In Vitro, Western Blot

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Long non-coding RNA P4713 contributes to the malignant phenotypes of oral squamous cell carcinoma by activating the JAK/STAT3 pathway

doi:

Figure Lengend Snippet: The relationship between P4713 and the JAK/STAT3 pathway. (A) Hierarchically clustered heatmaps of mRNAs altered in oral squamous cell carcinoma (OSCC; fold change > 2, P < 0.05). (B) The lncRNA-P4713 subnetwork in the OSCC co-expression network. (C) The top twenty GO terms of upregulated and downregulated mRNAs in OSCC cases (P < 0.05). (D) Significantly enriched pathways of the indicated gene sets. (E) qRT-PCR and (F) western blotting were used to measure the JAK/STAT3 pathway affected by P4713. (G) Representative fluorescent images of the location of STAT3. Scale bar = 10 µm.

Article Snippet: Microarray analysis The OSCC lncRNA/mRNA microarray analysis was performed by CapitalBio Corporation (Beijing, China).

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Journal: OncoTargets and therapy

Article Title: Long Non-Coding RNA NRAD1 and LINC00152 are Highly Expressed and Associated with Prognosis in Patients with Hepatocellular Carcinoma

doi: 10.2147/OTT.S251231

Figure Lengend Snippet: Relative expression of lncRNAs. ( A, B ) The raw images of the microarray analysis were shown; ( C ) the scatter plot was shown; ( D ) The comparison of relative expression of NRAD1 in HCC and normal cell lines; ( E ) The comparison of relative expression of LINC00152 in HCC and normal cell lines.

Article Snippet: We performed LncRNA and mRNA human gene expression microarray V4.0 (Capitalbio, 4×180K, two-channel, containing about 37 thousand lncRNAs and 34 thousand mRNAs) was applied to the profiling of lncRNAs in four cancerous tissues and the paired paracancerous tissues.

Techniques: Expressing, Microarray, Comparison